Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure.
Identifieur interne : 003C08 ( Main/Exploration ); précédent : 003C07; suivant : 003C09Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure.
Auteurs : Jean-Charles Leplé [Belgique] ; Rebecca Dauwe ; Kris Morreel ; Véronique Storme ; Catherine Lapierre ; Brigitte Pollet ; Annette Naumann ; Kyu-Young Kang ; Hoon Kim ; Katia Ruel ; Andrée Lefèbvre ; Jean-Paul Joseleau ; Jacqueline Grima-Pettenati ; Riet De Rycke ; Sara Andersson-Gunner S ; Alexander Erban ; Ines Fehrle ; Michel Petit-Conil ; Joachim Kopka ; Andrea Polle ; Eric Messens ; Björn Sundberg ; Shawn D. Mansfield ; John Ralph ; Gilles Pilate ; Wout BoerjanSource :
- The Plant cell [ 1040-4651 ] ; 2007.
Descripteurs français
- KwdFr :
- Aldehyde oxidoreductases (génétique), Analyse de profil d'expression de gènes (MeSH), Chromatographie en phase liquide à haute performance (MeSH), Fluorescence (MeSH), Glucides (MeSH), Immunohistochimie (MeSH), Lignine (composition chimique), Lignine (métabolisme), Paroi cellulaire (composition chimique), Paroi cellulaire (ultrastructure), Phénols (analyse), Phénotype (MeSH), Populus (cytologie), Populus (enzymologie), Populus (génétique), Populus (ultrastructure), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (génétique), Solubilité (MeSH), Spectroscopie infrarouge à transformée de Fourier (MeSH), Végétaux génétiquement modifiés (MeSH), Xylème (croissance et développement), Xylème (cytologie), Xylème (ultrastructure).
- MESH :
- analyse : Phénols.
- composition chimique : Lignine, Paroi cellulaire.
- croissance et développement : Xylème.
- cytologie : Populus, Xylème.
- enzymologie : Populus.
- génétique : Aldehyde oxidoreductases, Populus, Régulation négative.
- métabolisme : Lignine.
- Analyse de profil d'expression de gènes, Chromatographie en phase liquide à haute performance, Fluorescence, Glucides, Immunohistochimie, Paroi cellulaire, Phénotype, Populus, Régulation de l'expression des gènes végétaux, Solubilité, Spectroscopie infrarouge à transformée de Fourier, Végétaux génétiquement modifiés, Xylème.
English descriptors
- KwdEn :
- Aldehyde Oxidoreductases (genetics), Carbohydrates (MeSH), Cell Wall (chemistry), Cell Wall (ultrastructure), Chromatography, High Pressure Liquid (MeSH), Down-Regulation (genetics), Fluorescence (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Immunohistochemistry (MeSH), Lignin (chemistry), Lignin (metabolism), Phenols (analysis), Phenotype (MeSH), Plants, Genetically Modified (MeSH), Populus (cytology), Populus (enzymology), Populus (genetics), Populus (ultrastructure), Solubility (MeSH), Spectroscopy, Fourier Transform Infrared (MeSH), Xylem (cytology), Xylem (growth & development), Xylem (ultrastructure).
- MESH :
- chemical , analysis : Phenols.
- chemical , chemistry : Lignin.
- chemical , genetics : Aldehyde Oxidoreductases.
- chemical , metabolism : Lignin.
- chemical : Carbohydrates.
- chemistry : Cell Wall.
- cytology : Populus, Xylem.
- enzymology : Populus.
- genetics : Down-Regulation, Populus.
- growth & development : Xylem.
- ultrastructure : Cell Wall, Populus, Xylem.
- Chromatography, High Pressure Liquid, Fluorescence, Gene Expression Profiling, Gene Expression Regulation, Plant, Immunohistochemistry, Phenotype, Plants, Genetically Modified, Solubility, Spectroscopy, Fourier Transform Infrared.
Abstract
Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR in transgenic poplar (Populus tremula x Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration of the outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry of lignin epitopes, and oligolignol profiling indicated that lignin was relatively more reduced in syringyl than in guaiacyl units. The cohesion of the walls was affected, particularly at sites that are generally richer in syringyl units in wild-type poplar. Ferulic acid was incorporated into the lignin via ether bonds, as evidenced independently by thioacidolysis and by NMR. A synthetic lignin incorporating ferulic acid had a red-brown coloration, suggesting that the xylem coloration was due to the presence of ferulic acid during lignification. Elevated ferulic acid levels were also observed in the form of esters. Transcript and metabolite profiling were used as comprehensive phenotyping tools to investigate how CCR downregulation impacted metabolism and the biosynthesis of other cell wall polymers. Both methods suggested reduced biosynthesis and increased breakdown or remodeling of noncellulosic cell wall polymers, which was further supported by Fourier transform infrared spectroscopy and wet chemistry analysis. The reduced levels of lignin and hemicellulose were associated with an increased proportion of cellulose. Furthermore, the transcript and metabolite profiling data pointed toward a stress response induced by the altered cell wall structure. Finally, chemical pulping of wood derived from 5-year-old, field-grown transgenic lines revealed improved pulping characteristics, but growth was affected in all transgenic lines tested.
DOI: 10.1105/tpc.107.054148
PubMed: 18024569
PubMed Central: PMC2174873
Affiliations:
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Mansfield</name></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name></author><author><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name></author></analytic><series><title level="j">The Plant cell</title><idno type="ISSN">1040-4651</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aldehyde Oxidoreductases (genetics)</term><term>Carbohydrates (MeSH)</term><term>Cell Wall (chemistry)</term><term>Cell Wall (ultrastructure)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Down-Regulation (genetics)</term><term>Fluorescence (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Immunohistochemistry (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Phenols (analysis)</term><term>Phenotype (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (cytology)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (ultrastructure)</term><term>Solubility (MeSH)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>Xylem (cytology)</term><term>Xylem (growth & development)</term><term>Xylem (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aldehyde oxidoreductases (génétique)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Fluorescence (MeSH)</term><term>Glucides (MeSH)</term><term>Immunohistochimie (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Paroi cellulaire (composition chimique)</term><term>Paroi cellulaire (ultrastructure)</term><term>Phénols (analyse)</term><term>Phénotype (MeSH)</term><term>Populus (cytologie)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Populus (ultrastructure)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (génétique)</term><term>Solubilité (MeSH)</term><term>Spectroscopie infrarouge à transformée de Fourier (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Xylème (croissance et développement)</term><term>Xylème (cytologie)</term><term>Xylème (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aldehyde Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbohydrates</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Phénols</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Down-Regulation</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aldehyde oxidoreductases</term><term>Populus</term><term>Régulation négative</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Wall</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Fluorescence</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Immunohistochemistry</term><term>Phenotype</term><term>Plants, Genetically Modified</term><term>Solubility</term><term>Spectroscopy, Fourier Transform Infrared</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Chromatographie en phase liquide à haute performance</term><term>Fluorescence</term><term>Glucides</term><term>Immunohistochimie</term><term>Paroi cellulaire</term><term>Phénotype</term><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term><term>Solubilité</term><term>Spectroscopie infrarouge à transformée de Fourier</term><term>Végétaux génétiquement modifiés</term><term>Xylème</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR in transgenic poplar (Populus tremula x Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration of the outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry of lignin epitopes, and oligolignol profiling indicated that lignin was relatively more reduced in syringyl than in guaiacyl units. The cohesion of the walls was affected, particularly at sites that are generally richer in syringyl units in wild-type poplar. Ferulic acid was incorporated into the lignin via ether bonds, as evidenced independently by thioacidolysis and by NMR. A synthetic lignin incorporating ferulic acid had a red-brown coloration, suggesting that the xylem coloration was due to the presence of ferulic acid during lignification. Elevated ferulic acid levels were also observed in the form of esters. Transcript and metabolite profiling were used as comprehensive phenotyping tools to investigate how CCR downregulation impacted metabolism and the biosynthesis of other cell wall polymers. Both methods suggested reduced biosynthesis and increased breakdown or remodeling of noncellulosic cell wall polymers, which was further supported by Fourier transform infrared spectroscopy and wet chemistry analysis. The reduced levels of lignin and hemicellulose were associated with an increased proportion of cellulose. Furthermore, the transcript and metabolite profiling data pointed toward a stress response induced by the altered cell wall structure. Finally, chemical pulping of wood derived from 5-year-old, field-grown transgenic lines revealed improved pulping characteristics, but growth was affected in all transgenic lines tested.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18024569</PMID><DateCompleted><Year>2008</Year><Month>03</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1040-4651</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>11</Issue><PubDate><Year>2007</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The Plant cell</Title><ISOAbbreviation>Plant Cell</ISOAbbreviation></Journal><ArticleTitle>Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure.</ArticleTitle><Pagination><MedlinePgn>3669-91</MedlinePgn></Pagination><Abstract><AbstractText>Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR in transgenic poplar (Populus tremula x Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration of the outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry of lignin epitopes, and oligolignol profiling indicated that lignin was relatively more reduced in syringyl than in guaiacyl units. The cohesion of the walls was affected, particularly at sites that are generally richer in syringyl units in wild-type poplar. Ferulic acid was incorporated into the lignin via ether bonds, as evidenced independently by thioacidolysis and by NMR. A synthetic lignin incorporating ferulic acid had a red-brown coloration, suggesting that the xylem coloration was due to the presence of ferulic acid during lignification. Elevated ferulic acid levels were also observed in the form of esters. Transcript and metabolite profiling were used as comprehensive phenotyping tools to investigate how CCR downregulation impacted metabolism and the biosynthesis of other cell wall polymers. Both methods suggested reduced biosynthesis and increased breakdown or remodeling of noncellulosic cell wall polymers, which was further supported by Fourier transform infrared spectroscopy and wet chemistry analysis. The reduced levels of lignin and hemicellulose were associated with an increased proportion of cellulose. Furthermore, the transcript and metabolite profiling data pointed toward a stress response induced by the altered cell wall structure. Finally, chemical pulping of wood derived from 5-year-old, field-grown transgenic lines revealed improved pulping characteristics, but growth was affected in all transgenic lines tested.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Leplé</LastName><ForeName>Jean-Charles</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dauwe</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Morreel</LastName><ForeName>Kris</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Storme</LastName><ForeName>Véronique</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Lapierre</LastName><ForeName>Catherine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Pollet</LastName><ForeName>Brigitte</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Naumann</LastName><ForeName>Annette</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Kyu-Young</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hoon</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Ruel</LastName><ForeName>Katia</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Lefèbvre</LastName><ForeName>Andrée</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Joseleau</LastName><ForeName>Jean-Paul</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Grima-Pettenati</LastName><ForeName>Jacqueline</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>De Rycke</LastName><ForeName>Riet</ForeName><Initials>R</Initials></Author><Author 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PubStatus="entrez"><Year>2007</Year><Month>11</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18024569</ArticleId><ArticleId IdType="pii">tpc.107.054148</ArticleId><ArticleId IdType="doi">10.1105/tpc.107.054148</ArticleId><ArticleId IdType="pmc">PMC2174873</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant J. 2000 Jul;23(1):131-42</Citation><ArticleIdList><ArticleId IdType="pubmed">10929108</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2005 Jul;167(1):143-54</Citation><ArticleIdList><ArticleId IdType="pubmed">15948837</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Dec;136(4):4159-68</Citation><ArticleIdList><ArticleId IdType="pubmed">15557093</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2003 Oct 8;51(21):6178-83</Citation><ArticleIdList><ArticleId IdType="pubmed">14518941</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2001 Nov;28(3):271-82</Citation><ArticleIdList><ArticleId IdType="pubmed">11722770</ArticleId></ArticleIdList></Reference><Reference><Citation>Biostatistics. 2004 Jan;5(1):89-111</Citation><ArticleIdList><ArticleId IdType="pubmed">14744830</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2007 Oct;52(2):263-85</Citation><ArticleIdList><ArticleId IdType="pubmed">17727617</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2004 Aug;219(4):694-704</Citation><ArticleIdList><ArticleId IdType="pubmed">15146331</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2007 Apr;48(4):638-47</Citation><ArticleIdList><ArticleId IdType="pubmed">17317685</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2006 Jan;140(1):30-48</Citation><ArticleIdList><ArticleId IdType="pubmed">16377748</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 May 8;98(10):5916-21</Citation><ArticleIdList><ArticleId IdType="pubmed">11320207</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2003 Jan 3;278(1):95-103</Citation><ArticleIdList><ArticleId IdType="pubmed">12381722</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2005 Jul;46(7):1073-82</Citation><ArticleIdList><ArticleId IdType="pubmed">15870094</ArticleId></ArticleIdList></Reference><Reference><Citation>C R Biol. 2004 Sep-Oct;327(9-10):809-15</Citation><ArticleIdList><ArticleId IdType="pubmed">15587072</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1987 Jun 5;236(4806):1299-302</Citation><ArticleIdList><ArticleId IdType="pubmed">17770331</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2002 Jul;14(7):1557-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12119374</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Nov;136(3):3537-49</Citation><ArticleIdList><ArticleId IdType="pubmed">15516504</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8955-60</Citation><ArticleIdList><ArticleId IdType="pubmed">10430877</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1997 Mar;11(3):429-41</Citation><ArticleIdList><ArticleId IdType="pubmed">9107033</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1996 Dec;112(4):1479-1490</Citation><ArticleIdList><ArticleId IdType="pubmed">12226459</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Jan;53(2):368-79</Citation><ArticleIdList><ArticleId IdType="pubmed">18184422</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2003 Jun;217(2):218-28</Citation><ArticleIdList><ArticleId IdType="pubmed">12783329</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2004 Oct;94(4):497-505</Citation><ArticleIdList><ArticleId IdType="pubmed">15319227</ArticleId></ArticleIdList></Reference><Reference><Citation>Org Lett. 2000 Jul 27;2(15):2197-200</Citation><ArticleIdList><ArticleId IdType="pubmed">10930242</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1990 Apr;2(4):279-289</Citation><ArticleIdList><ArticleId IdType="pubmed">12354959</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1990;41:455-96</Citation><ArticleIdList><ArticleId IdType="pubmed">11543592</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2001 Sep;58(10):1399-417</Citation><ArticleIdList><ArticleId IdType="pubmed">11693522</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 May;34(3):351-62</Citation><ArticleIdList><ArticleId IdType="pubmed">12713541</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2003;54:519-46</Citation><ArticleIdList><ArticleId IdType="pubmed">14503002</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Dec;17(12):3337-49</Citation><ArticleIdList><ArticleId IdType="pubmed">16284305</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Dec;48(5):806-17</Citation><ArticleIdList><ArticleId IdType="pubmed">17092314</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2005 Nov;56(421):2807-19</Citation><ArticleIdList><ArticleId IdType="pubmed">16143717</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2005 Nov;59(5):753-69</Citation><ArticleIdList><ArticleId IdType="pubmed">16270228</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Dec;48(5):674-86</Citation><ArticleIdList><ArticleId IdType="pubmed">17092316</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2000 Nov 24;275(47):36899-909</Citation><ArticleIdList><ArticleId IdType="pubmed">10934215</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2005 Nov;171(3):1257-65</Citation><ArticleIdList><ArticleId IdType="pubmed">16085705</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1984 Nov 26;12(22):8711-21</Citation><ArticleIdList><ArticleId IdType="pubmed">6095209</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 May;126(1):145-55</Citation><ArticleIdList><ArticleId IdType="pubmed">11351078</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2000 Sep;124(1):31-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10982419</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1999 Jan;119(1):153-64</Citation><ArticleIdList><ArticleId IdType="pubmed">9880356</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:167-194</Citation><ArticleIdList><ArticleId IdType="pubmed">15012190</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2006 Apr;223(5):975-89</Citation><ArticleIdList><ArticleId IdType="pubmed">16292660</ArticleId></ArticleIdList></Reference><Reference><Citation>Crit Rev Biochem Mol Biol. 2003;38(4):305-50</Citation><ArticleIdList><ArticleId IdType="pubmed">14551235</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2004 Jun;219(2):338-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15067547</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2006 Mar 31;281(13):8843-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16421107</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1994 Oct;6(10):1401-14</Citation><ArticleIdList><ArticleId IdType="pubmed">7994174</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1992 Apr;11(3):137-41</Citation><ArticleIdList><ArticleId IdType="pubmed">24213546</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Dec;136(4):4023-36</Citation><ArticleIdList><ArticleId IdType="pubmed">15563622</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2007 Jul;25(7):759-61</Citation><ArticleIdList><ArticleId IdType="pubmed">17572667</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2001 Sep;47(1-2):239-74</Citation><ArticleIdList><ArticleId IdType="pubmed">11554475</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Jul;135(3):1447-56</Citation><ArticleIdList><ArticleId IdType="pubmed">15220467</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2003 Feb;15(2):523-31</Citation><ArticleIdList><ArticleId IdType="pubmed">12566589</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2005 Oct 15;391(Pt 2):409-15</Citation><ArticleIdList><ArticleId IdType="pubmed">15969652</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2005 Apr;137(4):1302-18</Citation><ArticleIdList><ArticleId IdType="pubmed">15749991</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2003 Nov;53(5):647-661</Citation><ArticleIdList><ArticleId IdType="pubmed">15010604</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1994 Mar 16;1205(1):1-18</Citation><ArticleIdList><ArticleId IdType="pubmed">8142473</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2000 Mar 3;275(9):6537-45</Citation><ArticleIdList><ArticleId IdType="pubmed">10692459</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Mar;134(3):1200-5</Citation><ArticleIdList><ArticleId IdType="pubmed">14976233</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2005 Oct;42(10):829-35</Citation><ArticleIdList><ArticleId IdType="pubmed">16098775</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2002 Jun;20(6):607-12</Citation><ArticleIdList><ArticleId IdType="pubmed">12042866</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2004 Dec;55(408):2483-93</Citation><ArticleIdList><ArticleId IdType="pubmed">15448172</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2000 Dec;12(12):2409-2424</Citation><ArticleIdList><ArticleId IdType="pubmed">11148287</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2001 Nov;28(3):257-70</Citation><ArticleIdList><ArticleId IdType="pubmed">11722769</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2000 Jul;123(3):853-67</Citation><ArticleIdList><ArticleId IdType="pubmed">10889235</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2005 Feb 28;579(6):1332-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15733837</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1996 Oct;112(2):493-502</Citation><ArticleIdList><ArticleId IdType="pubmed">12226405</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2002 Dec;43(12):1445-55</Citation><ArticleIdList><ArticleId IdType="pubmed">12514241</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1998 May;117(1):101-12</Citation><ArticleIdList><ArticleId IdType="pubmed">9576779</ArticleId></ArticleIdList></Reference><Reference><Citation>Physiol Plant. 2004 Feb;120(2):319-327</Citation><ArticleIdList><ArticleId IdType="pubmed">15032867</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2001 Jan;13(1):73-88</Citation><ArticleIdList><ArticleId IdType="pubmed">11158530</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2003 Nov;133(3):1051-71</Citation><ArticleIdList><ArticleId IdType="pubmed">14612585</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2002 Oct;11(5):495-503</Citation><ArticleIdList><ArticleId IdType="pubmed">12437080</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2002;3(3):REVIEWS3004</Citation><ArticleIdList><ArticleId IdType="pubmed">11897031</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1999 Oct;121(2):383-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10517829</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2006 Nov;62(4-5):697-714</Citation><ArticleIdList><ArticleId IdType="pubmed">17016741</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2001 Apr;26(2):205-16</Citation><ArticleIdList><ArticleId IdType="pubmed">11389761</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2001 Sep 28;276(39):36566-74</Citation><ArticleIdList><ArticleId IdType="pubmed">11429408</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2000 Mar;21(6):537-46</Citation><ArticleIdList><ArticleId IdType="pubmed">10758504</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Jan 11;18(1):203</Citation><ArticleIdList><ArticleId IdType="pubmed">2408008</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2003 Dec;8(12):576-81</Citation><ArticleIdList><ArticleId IdType="pubmed">14659706</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Oct 21;280(42):35554-61</Citation><ArticleIdList><ArticleId IdType="pubmed">16123046</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13951-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15353603</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14246-51</Citation><ArticleIdList><ArticleId IdType="pubmed">10588691</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1997 Jul;114(3):1123-33</Citation><ArticleIdList><ArticleId IdType="pubmed">9232887</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2004 Oct;16(10):2749-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15377757</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2005 May;221(2):243-54</Citation><ArticleIdList><ArticleId 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|clé= pubmed:18024569
|texte= Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18024569" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |